Increase of widespread A, B1 and D Escherichia coli clones producing a high diversity of CTX-M-types in a Portuguese hospital

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Carla Rodrigues (Author) Other; Elisabete Machado (Author) Other; Joao Pires (Author) Other; Helena Ramos (Author) Other; Angela Novais (Author) Other; Luisa Peixe (Author) FFUP

Journal

Title: Future Microbiology

Vol. 10

Pages: 1125-1131

ISSN: 1746-0913

Publisher: Future Medicine Ltd.

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 14 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-00G-KFN

DOI: 10.2217/fmb.15.38

Abstract (EN): Aim: To characterize temporal shifts in extended-spectrum -lactamases (ESBLs) and clones of clinical Escherichia coli isolates. Materials & methods: All ESBL-producing E. coli isolates from a Portuguese hospital (n = 112; June 2006-June 2007 and January-December 2010) were characterized by identification of phylogenetic groups, ESBL-types and virulence genes, XbaI-PFGE and MLST. Results: We observed a substantial increase in widespread E. coli clones from phylogroups A, B1 and D (e.g., ST10, ST23, ST117, ST155, ST648) producing mainly CTX-M-1, -14, -32 or SHV-12, along with a decrease in the proportion of the predominant CTX-M-15-producing B2-ST131 clone. Conclusion: The amplification of diverse CTX-M-producing A, B1 and D clonal complexes, which have been long identified in Portuguese nonclinical settings, unveils a role for these reservoirs in the landscape of ESBL-producing E. coli in the clinical setting.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7