Snapshot on carbapenemase-producing Pseudomonas aeruginosa and Acinetobacter baumannii in Bucharest hospitals reveals unusual clones and novel genetic surroundings for bla(OXA-23)

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Irina Gheorghe (Author) Other; Angela Novais (Author) Other; Filipa Grosso (Author) Other; Carla Rodrigues (Author) Other; Mariana Carmen Chifiriuc (Author) Other; Veronica Lazar (Author) Other; Luisa Peixe (Author) FFUP

Journal

Title: Journal of Antimicrobial Chemotherapy

Vol. 70

Pages: 1016-1020

ISSN: 0305-7453

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 22 Citations

Scientific classification

FOS: Medical and Health sciences > Health sciences

Other information

Authenticus ID: P-00A-B2W

DOI: 10.1093/jac/dku527

Abstract (EN): Objectives: The present study was designed to provide a snapshot on carbapenemase-producing Pseudomonas aeruginosa (n = 11) and Acinetobacter baumannii (n = 7) isolates in hospitalized patients (November 2011, January-March 2012) from two main hospitals in Bucharest, south Romania. Methods: Clonality among isolates was established by PFGE, MLST and Fourier transform infrared spectroscopy. Carbapenemases were screened by the Blue-Carba test, PCR and sequencing. Transferability of bla(OXA-23) was tested by conjugation and plasmid typing (number, size and identity) was assessed by S1-PFGE, replicon typing, hybridization and PCR mapping. Results: All P. aeruginosa isolates carried chromosomally located bla(VIM-2), associated with a common class 1 integron (aacA7-bla(VIM-2)) or an atypical configuration (aacA7-bla(VIM-2)-dfrB5-tniC). These isolates belonged to unusual lineages; mostly ST233 disseminated in one hospital unit, with ST364 and ST1074 also being detected. A. baumannii isolates carried bla(OXA-23) in Tn2008, which was found truncating a TnaphA6 transposon located in a common 60 kb GR6 (aci6) pABKp1-like conjugative plasmid in highly related CC92 clones (ST437, ST764 and ST765), where CC stands for clonal complex. Conclusions: Our results show the spread of VIM-2-producing P. aeruginosa and OXA-23-producing A. baumannii clinical isolates in two hospitals from Bucharest and highlight a peculiar population structure in this Eastern European country. Also, we demonstrate the dissemination of a common and conjugative aci6 pABKp1-like plasmid scaffold in different A. baumannii clones and we report the first known identification of Tnaph6-carrying pACICU2-like plasmids in Europe.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5