Determination of histamine in part wines and grape juices by ion-pair extraction and stable isotope dilution GC-MS

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Fernandes, JO (Author) FFUP; Ferreira, MA (Author) Other

Journal

Title: Chromatographia

Vol. 52

Pages: 77-81

ISSN: 0009-5893

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 12 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-ZJK

DOI: 10.1007/bf02490796

Abstract (EN): An isotope dilution, GC-MS method for the quantitation of histamine in wines and grape juices was assayed. This method includes isolation of histamine and its deuterated analogue [alpha,alpha,beta,beta-H-2(4)]histamine, used as the labelled internal standard, by ion-pair extraction with bis-2-ethylhexylphosphate, conversion to the volatile derivative: tris-pentafluorobenzyl-histamine, capillary GC separation and measurement of the abundance ratio of the (M-181)(+) ions from labelled and unlabelled derivatives. The method was successfully applied to the determination of histamine in several samples of Port wine, red and white table wine and grape juice. Data are given on detectability, linearity, repeatability and recovery.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5