A PCR assay to detect trace amounts of soybean in meat sausages

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Sonia Soares (Author) Other; Isabel Mafra (Author) FFUP; Joana S Amaral (Author) Other; Maria Beatriz P P Oliveira (Author) FFUP

Journal

Title: International Journal of Food Science and Technology

Vol. 45

Pages: 2581-2588

ISSN: 0950-5423

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 17 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-003-0JY

DOI: 10.1111/j.1365-2621.2010.02421.x

Abstract (EN): P>Soybean proteins are the most widely used source vegetable proteins in the meat industry because of several interesting characteristics. As soybean is included in the group of ingredients potentially allergenic, if not declared, it can be considered a hidden allergen, representing a potential risk to sensitised individuals. The aim of this work was to optimise and apply DNA-based techniques for soybean detection in meat products, as alternative to the currently used protein-based methods. The optimised polymerase chain reaction (PCR) protocol targeting the soybean lectin gene enabled the detection of the addition of 0.1% and 0.5% of hydrated textured protein, which corresponded to 0.01% and 0.06% (w/w) of soybean protein in unprocessed and heat-processed pork meats, respectively. The established PCR technique, when applied to commercial meat sausages (eighteen samples), confirmed the presence of soybean declared in nine samples and indicated the presence of soybean in four samples with no labelled information about soybean. Additionally, the event-specific PCR detection of Roundup Ready (R) soybean was also performed, enabling the detection of transgenic DNA in three samples.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8