Evaluation of the Reactivity of Sera from Patients with Systemic Lupus Erythematosus Against the Human MCP1

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Elsa Bronze da Rocha (Author) FFUP; Ana Novoa (Author) Other; Natercia Teixeira (Author) FFUP; Carlos Silva Vasconcelos (Author) ICBAS; Conceicao Cerveira (Author) Other; Joao C E Castro e Melo (Author) Other; Manuel Cirne Carvalho (Author) Other

Journal

Title: Journal of Clinical Immunology

Vol. 32

Pages: 721-728

ISSN: 0271-9142

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-00H-4MP

DOI: 10.1007/s10875-012-9665-4

Abstract (EN): This study evaluates metaphase chromosome protein 1 (MCP1), a nuclear antigen, as a diagnostic marker for systemic lupus erythematosus (SLE). Reactivity of sera from 114 Portuguese patients with autoimmune rheumatic disease or from healthy blood donors (HBD), against MCP1, produced in bacteria (bact-MCP1) or in its native form (native-MCP1), was determined by immunoblotting. Predictive and discriminative power of MCP1 reactivity for SLE diagnosis in disease-control groups was evaluated by logistic regression, its diagnostic value determined by receiver-operating characteristic analysis and compared with similar analysis of antinuclear antibody and double-stranded DNA (dsDNA). We demonstrated that native-MCP1, in contrast to bact-MCP1, reacts with SLE sera with significant predictive and discriminative power versus other autoimmune diseases (odds ratio [OR] a parts per thousand currency sign3.537 and a parts per thousand yen3.265; area under the receiver-operating characteristic curve [AUC] a parts per thousand currency sign0.643 and a parts per thousand yen0.636) or versus HBD (OR = 5.006; AUC = 0.671), showing a good diagnostic power with high specificity (82.1% versus HBD) and low sensitivity for SLE, similar to those of dsDNA. The reactivity of SLE sera with native-MCP1 was shown to be dependent on the presence of phosphorylated residues. Native-MCP1 was shown to have diagnostic value as a specific marker for SLE diagnosis and, therefore, is a suitable substrate for a new antibody test. The widely reported importance of phosphorylated epitopes as targets for autoantibodies in SLE could also be confirmed for native-MCP1.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8