Surface functionalization of polymeric nanospheres modulates macrophage activation: relevance in Leishmaniasis therapy

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Daniela Barros (Author) Other; Sofia A C Costa Lima (Author) Other; Anabela Cordeiro da Silva (Author) FFUP

Journal

Title: Nanomedicine

Vol. 10

Pages: 387-403

ISSN: 1743-5889

Publisher: Future Medicine Ltd.

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 29 Citations

Scientific classification

FOS: Engineering and technology > Nano-technology

Other information

Authenticus ID: P-00A-785

DOI: 10.2217/nnm.14.116

Abstract (EN): Aim: To characterize the production and application of carbohydrate functionalized poly(d,l-lactide-co-glycolide) (PLGA) nanospheres as immune-modulatory mediators in the treatment of visceral leishmaniasis (VL). Materials & methods: PLGA nanospheres were prepared by nanoprecipitation and surface functionalized with mannose, mannan or mannosamine moieties using a carbodiimide reaction. Flow cytometry and fluorescence microscopy revealed the interaction of these nanospheres with macrophages. Results: The nanocarriers were taken up by murine primary macrophages using clathrin-mediated endocytosis. Co-culture of macrophages with carbohydrate-functionalized nanospheres led to their activation and production of pro-inflammatory cytokines. One dose of amphotericin B-loaded on mannan-functionalized nanospheres resulted in an efficacy VL therapy. Conclusion: This approach provides a promising therapy for VL and a new therapeutic nanoplatform for obligate intracellular pathogens.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17