In vitro and in vivo evaluation of blood coagulation activation of polyvinyl alcohol hydrogel plus dextran-based vascular grafts

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Nuno Alexandre (Author) Other; Elisio Costa (Author) FFUP; Susana Coimbra (Author) Other; Alice Silva (Author) FFUP; Ascensao Lopes (Author) Other; Miguel Rodrigues (Author) Other; Marta Santos (Author) Other; Ana Colette Mauricio (Author) Other; Jose Domingos Santos (Author) FEUP; Ana Lucia Luis (Author) Other

Journal

Title: Journal of Biomedical Materials Research - Part A

Vol. 103 No. 1

Pages: 1366-1379

ISSN: 1549-3296

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 30 Citations

Scopus - 31 Citations

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

Other information

Authenticus ID: P-00G-1E9

DOI: 10.1002/jbm.a.35275

Abstract (EN): Polyvinyl alcohol hydrogel (PVA) is a water-soluble synthetic polymer that is commonly used in biomedical applications including vascular grafting. It was argued that the copolymerization of PVA with dextran (Dx) can result in improvement of blood-biomaterial interactions. The focus of this experimental study was to assess that interaction through an in vivo and in vitro evaluation of the coagulation system activation. The thrombogenicity of the copolymer was determined by quantification of platelet adhesion through the lactate dehydrogenase assay, determination of whole blood clotting time, and by quantification of platelet activation by flow cytometry. The thrombin-antithrombin complex blood levels were also determined. The obtained results for the in vitro assays suggested a non-thrombogenic profile for PVA/Dx. Additionally in vivo coagulation and hematological parameters were determined in an animal model after PVA/Dx vascular graft implantation. For coagulation homeostasis assessment, the intrinsic and extrinsic pathway's activation was determined by measuring prothrombin time (PT) and activated partial thromboplastin time (aPTT). Other markers of coagulation and inflammation activation including d-dimers, interleukin-6, and C-reactive protein were also assessed. The PVA/Dx copolymer tended to inhibit platelet adhesion/activation process and the contact activation process for coagulation. These results were also confirmed with the in vivo experiments where the measurements for APTT, interleukin-6, and C-reactive protein parameters were normal considering the species normal range of values. The response to those events is an indicator of the in vitro and in vivo hemocompatibility of PVA/Dx and it allows us to select this biomaterial for further preclinical trials in vascular reconstruction. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1366-1379, 2015.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14