Automatic miniaturized flow methodology with in-line solid-phase extraction for quinine determination in biological samples

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Margarida Vaz, AM (Author) Other; Andre R T S Araujo (Author) Other; Joao L M Santos (Author) FFUP; Jose L F C Lima (Author) FFUP; Lucia L M F S Saraiva (Author) FFUP

Journal

Title: Analytical Methods

Vol. 4

Pages: 1681-1686

ISSN: 1759-9660

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-002-9FZ

DOI: 10.1039/c2ay05818a

Abstract (EN): The present work describes an analytical platform based on a multipumping flow injection analysis (MPFS) technique combined with in-line solid-phase extraction (SPE). The flow network has been tested with the determination of quinine in biological samples using fluorometry as the detection technique. Amberlite XAD-4 resin has been used as a solid phase and the implementation of a pH control strategy resulted in a simple and environmental approach for the preconcentration of quinine. Two solenoid valves allowed the deviation of the flow towards the resin column to carry out SPE procedures. The influence of parameters such as concentration, flow rate and volume of the different solutions on the sensitivity and performance of the MPFS was studied. Dynamic calibration ranges (0.78-150 ng mL(-1)) for quinine determination were applied by using a variable sample volume (120-1000 mu L). The developed methodology provided high relative extraction recoveries from human urine samples (85-115%). The proposed automatic methodology turns out to be very efficient and sustainable compared to the available procedures and it could prove to be an attractive alternative tool to perform in-line sample pre-treatment and subsequent direct determination of relevant organic compounds in pharmaceutical and clinical analyses.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6