Structural characterization of functionalized gold nanoparticles for drug delivery in cancer therapy: a NMR based approach

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Sílvia C. Coelho (Author) FEUP; Maria Rangel (Author) Other; Maria C. Pereira (Author) FEUP; Manuel A. N. Coelho (Author) FEUP; Galya Ivanova (Author) Other

Journal

Title: Physical Chemistry Chemical Physics

Vol. 17 No. 29

Pages: 18971-18979

ISSN: 1463-9076

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 25 Citations

Scopus - 28 Citations

INSPEC

Scientific classification

FOS: Natural sciences > Chemical sciences

Associated Projects

NanoEFEct - Nanoparticle enhanced molecular Fluorescence-Endoscopy for detection of early stage colorectal adenocarcinoma

Other information

Authenticus ID: P-00G-D5C

DOI: 10.1039/c5cp02717a

Abstract (EN): In the present paper, we report results from a study of the structure and physicochemical properties of gold nanoparticles modified with poly(ethylene glycol) (PEG) designed for the drug delivery of the proteasome inhibitor Bortezomib (BTZ) in cancer therapy. A number of advanced analytical techniques were used to define important physicochemical characteristics such as composition, structure, surface properties, particle size and morphology. A new approach based on detailed NMR studies was employed to define specific intermolecular interactions and mechanisms of drug immobilization and location into surface modified gold nanoparticles (AuNPs). Particularly important information was gained from analysis of NMR spectroscopic parameters such as the spectral line shape, translation diffusion, the nuclear Overhauser effect (NOE) and spin-lattice relaxation (T-1). The results confirmed the coexistence of two different types of BTZ inclusion into polyethylene glycol coated gold nanoparticles: (i) association with the polymer chains by weak H-bonds and/or dipole-charge interactions and (ii) adsorption on the surface of the gold nanoparticles. The results allowed for determination of the overall structure of Bortezomib loaded PEG coated AuNPs, which is related to the therapeutic drug efficacy and activity in the treatment of cancer.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9