Synthesis of a Molecularly Imprinted Polymer for Melamine Analysis in Milk by HPLC with Diode Array Detection

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Leandro Figueiredo (Author) FEUP; Lúcia Santos (Author) FEUP; Arminda Alves (Author) FEUP

Journal

Title: Advances in Polymer Technology

Vol. 34 No. 4

Pages: 21506-21512

ISSN: 0730-6679

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 6 Citations

ISI Web of Science

Scopus - 8 Citations

INSPEC

Scientific classification

FOS: Engineering and technology

CORDIS: Technological sciences

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00G-MZJ

DOI: 10.1002/adv.21506

Abstract (EN): A molecularly imprinted polymer (MIP) for melamine was prepared by suspension polymerization, using melamine as a template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker. The optimal template/monomer and monomer/cross-linker ratios were obtained, being defined as 1/15 and 1/12, respectively. Molecular recognition properties and binding capacity toward melamine were evaluated by adsorption tests. Compared with the nonimprinted polymer, theimprinted polymer showed higher affinity for melamine with a dissociation constant (K-d) and a maximum binding concentration of 568.4 +/- 7.4 mol/L and 96.8 +/- 2.3 mol/g, respectively. Under optimal conditions, the imprinted polymer was used as a dispersive extraction sorbent to extract melamine from different milk samples, before high performance liquid chromatography with diode array detection (HPLC-DAD) analysis. The validated method presented a limit of detection of 0.6 mg/kg. From the analysis of six different milks, melamine was detected in two of the considered samples at a maximum concentration of 9.3 +/- 0.3 mg/kg.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7