Bacterial community variations in an alfalfa- rice rotation system revealed by 16S rRNA gene 454-pyrosequencing

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Ana R. Lopes (Author) FEUP; Célia M. Manaia (Author) Other; Olga C. Nunes (Author) FEUP

Journal

Title: FEMS Microbiology Ecology

Vol. 87 No. 3

Pages: 650-663

ISSN: 0168-6496

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 35 Citations

Scopus - 55 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Associated Projects

UI0511 - PROJECTO ESTRATÉGICO - UI 511 - 2013-2014

Other information

Authenticus ID: P-009-KC4

DOI: 10.1111/1574-6941.12253

Abstract (EN): Crop rotation is a practice harmonized with the sustainable rice production. Nevertheless, the implications of this empirical practice are not well characterized, mainly in relation to the bacterial community composition and structure. In this study, the bacterial communities of two adjacent paddy fields in the 3rd and 4th year of the crop rotation cycle and of a nonseeded subplot were characterized before rice seeding and after harvesting, using 454-pyrosequencing of the 16S rRNA gene. Although the phyla Acidobacteria, Proteobacteria, Chloroflexi, Actinobacteria and Bacteroidetes predominated in all the samples, there were variations in relative abundance of these groups. Samples from the 3rd and 4th years of the crop rotation differed on the higher abundance of groups of presumable aerobic bacteria and of presumable anaerobic and acidobacterial groups, respectively. Members of the phylum Nitrospira were more abundant after rice harvest than in the previously sampled period. Rice cropping was positively correlated with the abundance of members of the orders Acidobacteriales and Solibacterales' and negatively with lineages such as Chloroflexi Ellin6529'. Studies like this contribute to understand variations occurring in the microbial communities in soils under sustainable rice production, based on real-world data.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14