Studies on the properties of Celluclast/Eudragit L-100 conjugate

• Type: Article in International Scientific Journal
• Date: 2002

Authors

Dourado, F (Author) Other; Bastos, M (Author) FCUP; Mota, M (Author) Other; Gama, FM (Author) Other

Journal

Title: Journal of Biotechnology

Vol. 99

Pages: 121-131

ISSN: 0168-1656

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 46 Citations

Scopus - 50 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-MNK

DOI: 10.1016/s0168-1656(02)00178-5

Abstract (EN): A cellulase from Trichoderma reesei was immobilized on Eudragit L-100, a reversibly soluble polymer depending on the pH of the medium. The solubility of the modified cellulase was studied at different pH values. By changing the pH, the adsorption equilibrium of the derivatized proteins is switched towards the liquid phase, thus making recycling possible. This method allows for improved stability, without major loss of specific activity. The adsorption of cellulase on Eudragit lowers the enthalpy of denaturation, but affects only slightly the denaturation temperature. The use of carbodiimide was ineffective on linking the enzymes covalently to the polymer, since the immobilization process was found to be only mediated by non-covalent forces.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11