Caenibacterium thermophilum gen. nov., sp nov., isolated from a thermophilic aerobic digester of municipal sludge

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Célia M. Manaia (Author) Other; Olga C. Nunes (Author) FEUP; Balbina Nogales (Author) Other

Journal

Title: International Journal of Systematic and Evolutionary Microbiology

Vol. 53 No. Part: 5

Pages: 1375-1382

ISSN: 1466-5026

Publisher: Microbiology Society

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 17 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-FAY

DOI: 10.1099/ijs.0.02622-0

Abstract (EN): A bacterial strain, N2-680(T)( DSM 15264(T)-LMG 21 760(T)), isolated from a thermophilic aerobic digester of municipal sludge, was characterized with respect to its morphology, physiology and taxonomy. Phenotypically, the isolate was a Gram-negative rod with a polar flagellum, catalase- and oxidase- positive, containing cytoplasmic inclusions of poly-beta-hydroxybutyrate and had an optimal growth temperature of about 47 degreesC. Strain N2-680(T) was unable to reduce nitrate and could use organic acids, amino acids and carbohydrates as single carbon sources. Chemotaxonomic analysis revealed that ubiquinone 8 was the major respiratory quinone of this organism and that phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. At 50 degreesC the major components in fatty acid methyl ester analysis were C-16:0 and cyclo-C-17: 0. The highest 16S rDNA sequence identity of isolate N2-680(T) was to Leptothrix mobilis and Ideonella dechloratans (95.7 %) and to Rubrivivax gelatinosus. and Aquabacterium commune (95.6 %). 16S rDNA sequence similarities to species of two related thermophilic genera, Caldimonas manganoxidans and Tepidimonas ignava, were lower (93.6 and 94.7 %). On the basis of phylogenetic analyses and physiological and chemotaxonomic characteristics, it is proposed that isolate N2-680(T) represents a new genus and species, for which the name Caenibacterium thermophilum gen. nov., sp. nov. is proposed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8