Time to "go large" on biofilm research: advantages of an omics approach

• Type: Another Publication in an International Scientific Journal
• Date: 2009

Authors

Nuno F Azevedo (Author) FEUP; Susana P Lopes (Author) Other; Charles W Keevil (Author) Other; Maria O Pereira (Author) Other; Maria J Vieira (Author) Other

Journal

Title: Biotechnology Letters

Vol. 31

Pages: 477-485

ISSN: 0141-5492

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 16 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-003-KZ2

DOI: 10.1007/s10529-008-9901-4

Abstract (EN): In nature, the biofilm mode of life is of great importance in the cell cycle for many microorganisms. Perhaps because of biofilm complexity and variability, the characterization of a given microbial system, in terms of biofilm formation potential, structure and associated physiological activity, in a large-scale, standardized and systematic manner has been hindered by the absence of high-throughput methods. This outlook is now starting to change as new methods involving the utilization of microtiter-plates and automated spectrophotometry and microscopy systems are being developed to perform large-scale testing of microbial biofilms. Here, we evaluate if the time is ripe to start an integrated omics approach, i.e., the generation and interrogation of large datasets, to biofilms-"biofomics". This omics approach would bring much needed insight into how biofilm formation ability is affected by a number of environmental, physiological and mutational factors and how these factors interplay between themselves in a standardized manner. This could then lead to the creation of a database where biofilm signatures are identified and interrogated. Nevertheless, and before embarking on such an enterprise, the selection of a versatile, robust, high-throughput biofilm growing device and of appropriate methods for biofilm analysis will have to be performed. Whether such device and analytical methods are already available, particularly for complex heterotrophic biofilms is, however, very debatable.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9