Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH

• Type: Article in International Scientific Journal
• Date: 2013

Authors

C. Almeida (Author) FEUP; N. F. Azevedo (Author) FEUP; J. C. Bento (Author) Other; N. Cerca (Author) Other; H. Ramos (Author) Other; M. J. Vieira (Author) Other; C. W. Keevil (Author) Other

Journal

Title: European Journal of Clinical Microbiology and Infectious Diseases

Vol. 32 No. 6

Pages: 781-786

ISSN: 0934-9723

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 11 Citations

Scientific classification

FOS: Medical and Health sciences > Health sciences

Associated Projects

DNA mimics - Desenvolvimento e aplicação de mímicos de DNA para a rápida identificação de microrganismos patogénicos

Other information

Authenticus ID: P-005-0BM

DOI: 10.1007/s10096-012-1808-2

Abstract (EN): We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2-99.7) and 100 % (95 % CI, 80,8-100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6