Implanted neonatal human dermal fibroblasts influence the recruitment of endothelial cells in mice.

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Guerreiro, SG (Author) Other; Brochhausen, C (Author) Other; Negrao, R (Author) Other; Barbosa, MA (Author) Other; Unger, RE (Author) Other; Kirkpatrick, CJ (Author) Other; Soares, R (Author) Other; Granja, PL (Author) FEUP

Journal

Title: Biomatter

Vol. 2

Pages: 43-52

ISSN: 2159-2527

Publisher: Landes Bioscience

Indexing

ISI Web of Knowledge

Scopus - 15 Citations

Other information

Authenticus ID: P-00G-2Z2

DOI: 10.4161/biom.20063

Abstract (EN): The vascularization of new tissue within a reasonable time is a crucial prerequisite for the success of different cell- and material-based strategies. Considering that angiogenesis is a multi-step process involving humoral and cellular regulatory components, only in vivo assays provide the adequate information about vessel formation and the recruitment of endothelial cells. The present study aimed to investigate if neonatal human dermal fibroblasts could influence in vivo neovascularization. Results obtained showed that fibroblasts were able to recruit endothelial cells to vascularize the implanted matrix, which was further colonized by murine functional blood vessels after one week. The vessels exhibited higher levels of hemoglobin, compared with the control matrix, implanted without fibroblasts, in which no vessel formation could be observed. No significant differences were detected in systemic inflammation. The presence of vessels originated from the host vasculature suggested that host vascular response was involved, which constitutes a fundamental aspect in the process of neovascularization. Fibroblasts implanted within matrigel increased the presence of endothelial cells with positive staining for CD31 and for CD34 and the production of collagen influencing the angiogenic process and promoting the formation of microvessels. New strategies in tissue engineering could be delineated with improved angiogenesis using neonatal fibroblasts.

Language: English

Type (Professor's evaluation): Scientific