Effect of Cell Density on Mesenchymal Stem Cells Aggregation in RGD-Alginate 3D Matrices under Osteoinductive Conditions

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Raquel Maia, FR (Author) Other; Ana H Lourenco (Author) Other; Pedro L Granja (Author) FEUP; Raquel M Goncalves (Author) Other; Cristina C Barrias (Author) Other

Journal

Title: Macromolecular Bioscience

Vol. 14

Pages: 759-771

ISSN: 1616-5187

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 49 Citations

Scopus - 53 Citations

Other information

Authenticus ID: P-009-JKG

DOI: 10.1002/mabi.201300567

Abstract (EN): Cellular activities in 3D are differentially affected by several matrix-intrinsic and extrinsic factors. This study highlights the relevance of optimizing initial cell densities when establishing 3D cultures for specific applications. Independently of the entrapping density, MSCs cultured within RGD-alginate hydrogels showed steady-state levels of metabolic activity and were in a nearly non-proliferative state, but recovered "normal'' activity levels when retrieved from 3D matrices and re-cultured as monolayers. Importantly, high-densities promoted the establishment of cell-cell contacts with formation of multicellular clusters stabilized by endogenous ECM, and also stimulated MSCs osteogenic differentiation. These MSC-ECM microtissues may be used as building blocks for tissue engineering.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13