Molecular Interaction of Rifabutin on Model Lung Surfactant Monolayers

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Marina Pinheiro (Author) Other; Marlene Lucio (Author) FFUP; Salette Reis (Author) FFUP; Jose L F C Lima (Author) FFUP; Joao M Caio (Author) Other; Cristina Moiteiro (Author) Other; Maria T Martin Romero (Author) Other; Luis Camacho (Author) Other; Juan J Giner Casares (Author) Other

Journal

Title: Journal of Physical Chemistry B

Vol. 116 No. 6

Pages: 11635-11645

ISSN: 1520-6106

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 11 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-002-5CD

DOI: 10.1021/jp303725j

Abstract (EN): Tuberculosis is one of the most relevant problems for global health care. The design of new drugs against tuberculosis is aimed at maximizing impact against the disease, as well as minimizing the toxicological effect on the lung surfactant. In this work, the antituberculosis drug Rifabutin is studied in combination with phospholipid Langmuir monolayers as models of the lung surfactant monolayer. The zwitterionic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and the anionic 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DPPG) were used as model phospholipids. A combination of in situ experimental techniques of Brewster angle microscopy, polarization-modulated infrared reflection-absorption spectroscopy, and UV-vis reflection spectroscopy with computer simulations has been used. The interactions between Rifabutin and the DPPC and DPPG Langmuir monolayers were described as the formation of an inclusion complex. The phospholipid Rifabutin inclusion complex prevents the penetration of the Rifabutin into the alkyl chain region of the phospholipids, leading to a disruption of the monolayer structure and a possible toxicological effect.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11