Determination of scavenging capacity against hydrogen peroxide: Recent trends on chemical methods

• Type: Chapter or Part of a Book
• Date: 2013

Authors

Segundo, MA (Author) FFUP; Castro, C (Author) Other; Magalhaes, LM (Author) Other; Reis, S (Author) FFUP

Book

Title: Hydrogen Peroxide: Detection, Applications and Health Implications

Pages: 203-218

ISBN: 9781622574148

Indexing

ISI Web of Knowledge

Scopus - 2 Citations

Other information

Authenticus ID: P-008-YMZ

Abstract (EN): Hydrogen peroxide is one of the most important reactive oxygen species and it has gained an enormous attention due to its high permeability within cell and across cellular membranes, as an in vivo precursor of strong oxidants (namely hydroxyl radical and hypochlorous acid) and due to its implication in the generation of superoxide radical anion. Therefore, the development of reliable analytical methods to determine the scavenging capacity against hydrogen peroxide has been a topic of increased interest within pharmaceutical and biomedical area. Currently, several methods are available for performing the determination of scavenging capacity against hydrogen peroxide using UV-vis spectrophotometry, fluorescence and chemiluminescence as detection systems. In this chapter, a critical review of these methods will be undertaken, by comparing the figures of merit and limitations provided by some of the most applied methods (measurement of intrinsic absorption of H 2 O 2 in UV region, fluorimetric assays based in scopoletin and homovanillic acid, chemiluminescence quenching of luminol and lucigenin, among others). Moreover, special emphasis will be given to methods developed in the last three years, based on automatic systems and nanotechnology breakthroughs.

Language: English

Type (Professor's evaluation): Scientific