Determination of the scavenging capacity against reactive nitrogen species by automatic flow injection-based methodologies.

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Segundo, MA (Author) FFUP; Magalhaes, LM (Author) Other; Ribeiro, JP (Author) Other; Lucio, M (Author) FEUP; Reis, S (Author) FFUP

Journal

Title: Methods in molecular biology (Clifton, N.J.)

Vol. 704

Pages: 91-104

ISSN: 1064-3745

Publisher: Springer Nature

Indexing

ISI Web of Knowledge

Scopus - 1 Citation

Other information

Authenticus ID: P-005-4C7

DOI: 10.1007/978-1-61737-964-2_8

Abstract (EN): Automatic flow-based systems have been applied to assay scavenging capacity against reactive oxygen and nitrogen species, providing analytical tools which can cope with different types and large number of samples. In the present chapter, a flow injection analysis procedure is described for the assessment of peroxynitrite scavenging. A sequential injection analysis procedure is also described for determining the scavenging capacity against the nitric oxide radical. For both systems, reaction between putative antioxidants and the reactive species of nitrogen takes place inside the flow conduits before addition of luminol and further detection of remaining reactive species by chemiluminescence.

Language: English

Type (Professor's evaluation): Scientific