Phlorotannin Extracts from Fucales Characterized by HPLC-DAD-ESI-MSn: Approaches to Hyaluronidase Inhibitory Capacity and Antioxidant Properties

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Federico Ferreres (Author) Other; Graciliana Lopes (Author) Other; Angel Gil Izquierdo (Author) Other; Paula B Andrade (Author) FFUP; Carla Sousa (Author) FFUP; Teresa Mouga (Author) Other; Patricia Valentao (Author) FFUP

Journal

Title: Marine Drugs

Vol. 10 No. 10

Pages: 2766-2781

ISSN: 1660-3397

Publisher: MDPI

Indexing

ISI Web of Knowledge - 63 Citations

ISI Web of Science

Scopus - 121 Citations

Scientific classification

CORDIS: Health sciences

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-002-2RF

DOI: 10.3390/md10122766

Abstract (EN): Purified phlorotannin extracts from four brown seaweeds (Cystoseira nodicaulis (Withering) M. Roberts, Cystoseira tamariscifolia (Hudson) Papenfuss, Cystoseira usneoides (Linnaeus) M. Roberts and Fucus spiralis Linnaeus), were characterized by HPLC-DAD-ESI-MSn. Fucophloroethol, fucodiphloroethol, fucotriphloroethol, 7-phloroeckol, phlorofucofuroeckol and bieckol/dieckol were identified. The antioxidant activity and the hyaluronidase (HAase) inhibitory capacity exhibited by the extracts were also assessed. A correlation between the extracts activity and their chemical composition was established. F. spiralis, the species presenting higher molecular weight phlorotannins, generally displayed the strongest lipid peroxidation inhibitory activity (IC50 = 2.32 mg/mL dry weight) and the strongest HAase inhibitory capacity (IC50 = 0.73 mg/mL dry weight). As for superoxide radical scavenging, C. nodicaulis was the most efficient species (IC50 = 0.93 mg/mL dry weight), followed by F. spiralis (IC50 = 1.30 mg/mL dry weight). These results show that purified phlorotannin extracts have potent capabilities for preventing and slowing down the skin aging process, which is mainly associated with free radical damage and with the reduction of hyaluronic acid concentration, characteristic of the process.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16