Fast determination of bioactive compounds from Lycopersicon esculentum Mill. leaves

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Marcos Taveira (Author) Other; Federico Ferreres (Author) Other; Angel Gil Izquierdo (Author) Other; Luisa Oliveira (Author) Other; Patricia Valentao (Author) FFUP; Paula B Andrade (Author) FFUP

Journal

Title: Food Chemistry

Vol. 135 No. 3

Pages: 748-755

ISSN: 0308-8146

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 19 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-002-3EB

DOI: 10.1016/j.foodchem.2012.05.016

Abstract (EN): Lycopersicon esculentum leaves, usually considered as a by-product of tomato production, present several bioactive compounds of interest for industries like food, pharmaceutical and cosmetics. Nevertheless, before industrial application, suitable methods to identify and quantify those metabolites should be developed. In this study agitation with aqueous methanol was used for phenolic compounds extraction. Solid-phase extraction (SPE) was performed as the purification step before alkaloids analysis. Among the SPE sorbents tested, sulphonic acid bonded silica with H+ counterion (SCX) proved to be the most efficient one for removing interfering components. Fifteen phenolics and four steroidic alkaloids were identified in 35 and 20 min analysis, respectively. The optimised methods were validated, revealing to be accurate, fast, simple and sensitive. Thus, these methods represent an easy and fast analytical approach, using equipment available in almost laboratory, which render them to be appropriate for routine analysis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8