Discrimination of single-porin Escherichia (E.) coli mutants by ATR and transmission mode FTIR spectroscopy

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Raul G Saraiva (Author) Other; Joao Almeida Lopes (Author) Other; Jorge Machado (Author) Other; Paula Gameiro (Author) FCUP; Maria J Feio (Author) Other

Journal

Title: Journal of Biophotonics

Vol. 7

Pages: 392-400

ISSN: 1864-063X

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 11 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-009-GR3

DOI: 10.1002/jbio.201200131

Abstract (EN): Vibrational spectroscopy has long been used in bacterial identification with different levels of taxonomic discrimination but its true potential for intra-species differentiation remains poorly explored. Herein, both transmission Fourier-transform infrared (FTIR) and attenuated total reflectance (ATR)-FTIR spectroscopy are used to analyse E. coli strains that differ solely in their porin expression profile. In this previously unreported approach, the applicability of both FTIR-spectroscopy techniques is compared with the same collection of unique strains. ATR-FTIR spectroscopy proved to reliably distinguish between several E. coli porin mutants with an accuracy not replicated by FTIR in transmission mode (using previously optimized procedures). Further studies should allow the identification of the individual contribution of the single porin channel to the overall bacterial infrared spectrum and of molecular predictive patterns of porin alterations. [GRAPHICS] Model of the Gram-negative bacteria envelope [adapted from V. Teixeira, M. J. Feio and M. Bastos, Prog Lipid Res. 51(2), 149-177 (2012)].

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9