Measuring beyond the microscope field of view using digital images in squamous cell carcinoma of the lip

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Cruz, D (Author) Other; Melo, N (Author) Other; Seixas, M (Author) Other; Lopes, JM (Author) FMUP

Journal

Title: Analytical and Quantitative Cytology and Histology

Vol. 23

Pages: 245-250

ISSN: 0884-6812

Publisher: Science Printers and Publishers

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-TND

Abstract (EN): OBJECTIVE: To study the impact of using digital images for measuring the size of the tumors, assisting with the prognostic evaluation of carcinomas of the oral mucosa. STUDY DESIGN: The depth of invasion of 12 cases of squamous cell carcinoma of the lower lip was assessed through a microscope and through digital images. All measurements of depth of invasion were assessed in a direction orthogonal to the lip surface. First, assessment of depth of invasion was done at the microscope, using an eyepiece reticule with an engraved scale. Second, depth of invasion was assessed by digital images, using a program module developed to assist pathologists with linear measuring. When the depth of the tumor was larger than the field of view at the proper magnification, several images were taken to include the whole area of invasion. The images were finally mounted in a single image and the depth of invasion measured. RESULTS: The results show positive and negative differences between assessments when assessing depths of < 2 mm. At greater depths ( greater than or equal to 2 mm), the difference was always negative, showing that for deep invasion, measurements of longer distances were always performed on digital images. CONCLUSION: Measurements with digital images beyond the field of view at proper magnification could significantly alter the diagnostic and prognostic assessment made using the microscope.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6