Using yeast to uncover the regulation of protein kinase C delta by ceramide

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Claudia Bessa (Author) Other; Clara Pereira (Author) Other; Mariana Leao (Author) Other; Claudia Maciel (Author) Other; Sara Gomes (Author) Other; Jorge Goncalves (Author) FFUP; Manuela Corte Real (Author) Other; Vitor Costa (Author) ICBAS; Lucilia Saraiva (Author) FFUP

Journal

Title: FEMS Yeast Research

Vol. 13

Pages: 700-705

ISSN: 1567-1356

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 3 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-006-JKZ

DOI: 10.1111/1567-1364.12069

Abstract (EN): The regulation of protein kinase C (PKC) isoforms by ceramide is still controversial. In this work, the yeast Saccharomyces cerevisiae was used as a model to elucidate the effect of ceramide on the activity of mammalian PKC isoforms. For that, isc1 cells, with a deletion in the pathway for ceramide production by hydrolysis of complex sphingolipids, individually expressing mammalian PKC, and were used. Contrary to PKC and , expression of PKC in isc1 cells exhibited a similar phenotype to that observed with wild-type yeast cells expressing PKC treated with a PKC activator, as phorbol 12-myristate 13-acetate (PMA), specifically a growth inhibition associated with a G2/M cell cycle arrest. Interestingly, in isc1 yeast cells expressing PKC this phenotype was completely abrogated in the presence of exogenous ceramide. Moreover, using a yeast-based assay previously developed for the screening of PKC inhibitors, it was also shown that, like the known PKC inhibitor NPC 15437, ceramide reduced the PMA-induced growth inhibition, supporting an inhibitory effect of ceramide on PKC. Altogether, these results may indicate that ceramide distinctly interfere with the activity of PKC, and . Most importantly, they showed that ceramide is an inhibitor of PKC.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6