Box-Behnken factorial design to obtain a phenolic-rich extract from the aerial parts of Chelidonium majus L.

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Clara Grosso (Author) Other; Federico Ferreres (Author) Other; Angel Gil Izquierdo (Author) Other; Patricia Valentao (Author) FFUP; Maria Sampaio (Author) Other; Julio Lima (Author) Other; Paula B Andrade (Author) FFUP

Journal

Title: Talanta

Vol. 130 No. 1

Pages: 128-136

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 28 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-009-QBW

DOI: 10.1016/j.talanta.2014.06.043

Abstract (EN): A Box-Behnken design (BBD) was developed to study the influence of four parameters (Xi: % methanol; X-2: extraction time; X-3: extraction temperature; X-4: solid/solvent ratio) on two responses, namely extraction yield and phenolics content of the aerial parts of Chelidonium majus L The model presented a good fit to the experimental results for the extraction yield, being significantly influenced by Xi and X-4. On the other hand a parameter reduction was necessary to run the model for phenolics content, showing that only X-1 and X-2 had great influence on the response. Two best extraction conditions were defined: X-1=76.8% MeOH, X-2=150.0 min, X-3=60.0 degrees C and X-4=1 :100 and X-1=69.2%, X-2=150 min, X-3=42.5 degrees C and X-4=1:100. Moreover, the HPLC-DAD-ESI/MSn analysis conducted with the center point sample revealed the presence of 15 alkaloids and 15 phenolic compounds, from which the 9 flavonoids and 3 hydroxycinnamic acids are described for the first time. Only phenolic compounds were quantified by a validated HPLC-DAD method, the pair quercetin-3-O-rutinoside+quercetin-3-O-glucoside dominating all the 29 extracts. This study is of great importance for future works that seek to apply the phenolic profile to the quality control of C. majus samples.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9