HPLC-DAD-ESI/MSn analysis of phenolic compounds for quality control of Grindelia robusta Nutt. and bioactivities

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Federico Ferreres (Author) Other; Clara Grosso (Author) Other; Angel Gil Izquierdo (Author) Other; Patricia Valentao (Author) FFUP; Carolina Azevedo (Author) Other; Paula B Andrade (Author) FFUP

Journal

Title: Journal of Pharmaceutical and Biomedical Analysis

Vol. 94

Pages: 163-172

ISSN: 0731-7085

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 17 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-009-HXP

DOI: 10.1016/j.jpba.2014.01.046

Abstract (EN): The phenolic composition of herbal tea (HT) and hydromethanolic extract (HME) obtained from Grindelia robusta Nutt. was studied by HPLC-DAD-ESI/MSn. Thirty six flavonoids and hydroxycinnamic acids were detected, from which thirty are described for the first time in this species. Quantification by HPLC-DAD showed that diosmetin-7-0-glucuronide-3'-0-pentoside + apigenin-7-0-glucuronide-4'-0-pentoside, apigenin-7-0-glucuronide + diosmetin-7-0-glucuronide and 3,5-dicaffeoylquinic acid + 3,4-dicaffeoylquinic acid were the major compounds. Since the health-promoting effects of natural phenolic compounds against brain disorders is of increasing interest, HT and HME were also tested against oxygen and nitrogen reactive species and against enzymes related with Alzheimer's disease and depression. Extracts displayed strong in vitro scavenging activity and monoamine oxidase-A (MAO-A) inhibitory activity. The anti-MAO-A capacity was observed at non-toxic concentrations for SH-SY5Y human neuroblastoma cell line, reinforcing the benefits of G. robusta HT. However, no protection against hydrogen peroxide treatment was observed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10