Quantification of free circulating tumor DNA as a diagnostic marker for breast cancer

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Raquel Catarino (Author) Other; Maria M Ferreira (Author) Other; Helena Rodrigues (Author) Other; Ana Coelho (Author) Other; Ana Nogal (Author) Other; Abreu Sousa (Author) Other; Rui Medeiros (Author) ICBAS

Journal

Title: DNA and Cell Biology

Vol. 27

Pages: 415-421

ISSN: 1044-5498

Publisher: Mary Ann Liebert

Indexing

ISI Web of Knowledge - 98 Citations

Scopus - 100 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-003-X6M

DOI: 10.1089/dna.2008.0744

Abstract (EN): Aim: To determine whether the amounts of circulating DNA could discriminate between breast cancer patients and healthy individuals by using real-time PCR quantification methodology. Methods: Our standard protocol for quantification of cell-free plasma DNA involved 175 consecutive patients with breast cancer and 80 healthy controls. Results: We found increased levels of circulating DNA in breast cancer patients compared to control individuals (105.2 vs. 77.06 ng/mL, p < 0.001). We also found statistically significant differences in circulating DNA amounts in patients before and after breast surgery ( 105.2 vs. 59.0 ng/mL, p 0.001). Increased plasma cell-free DNA concentration was a strong risk factor for breast cancer, conferring an increased risk for the presence of this disease ( OR, 12.32; 95% CI, 2.09-52.28; p < 0.001). Conclusions: Quantification of circulating DNA by real-time PCR may be a good and simple tool for detection of breast cancer with a potential to clinical applicability together with other current methods used for monitoring the disease.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7