Optimization and validation of a method based in a QuEChERS procedure and gas chromatography-mass spectrometry for the determination of multi-mycotoxins in popcorn

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Ferreira, I (Author) FFUP; Fernandes, JO (Author) FFUP; Cunha, SC (Author) Other

Journal

Title: Food Control

Vol. 27

Pages: 188-193

ISSN: 0956-7135

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 38 Citations

Scopus - 43 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-002-6S3

DOI: 10.1016/j.foodcont.2012.03.014

Abstract (EN): In this work a gas chromatography-mass spectrometry (GC-MS) method for multi-mycotoxins (deoxynivalenol, nivalenol, 15-acetyl-deoxynivalenol, fusarenon X and zearalenone) determination in unpopped and popped popcorn was optimized and validated. Fast sample preparation procedure is based on a pre-extraction of fat compounds with n-hexane, extraction of target analytes with acetonitrile and removing of matrix co-extracts by a simple dispersive solid phase extraction employing MgSO4 and C18 sorbents followed by sylilation of the analytes and further GC-MS analysis. The recoveries in spiked samples (20 and 100 mu g/kg) ranged from 61 to 118% in unpopped popcorn and from 65 to 89% in popped popcorn, with relative standard deviations lower than 18%. Limits of detection and quantification were lower than 65 mu g/kg and 196 mu g/kg, respectively. The method was applied in 30 popcorn samples commercialized in Portugal; one sample was positive for nivalenol and zearalenone, at concentrations below the maximum permissible level.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6