A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Rosa Pereira (Author) Other; Tommy Julianto (Author) Other; Peh Kim Ang (Author) Other; Sharon Sheue Nee Ling (Author) Other; Carlos Mauricio Barbosa (Author) FFUP; Kah Hay Yuen (Author) Other; Abu Bakar Abdul Majeed (Author) Other

Journal

Title: Chromatographia

Vol. 71 No. 5/6

Pages: 373-381

ISSN: 0009-5893

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 3 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-003-8G6

DOI: 10.1365/s10337-010-1481-5

Abstract (EN): A sensitive and rapid routine LC method was validated for measuring cefotaxime incorporated in three different pH-sensitive nanoparticles. The drug was chromatographed on a C18 reversed-phase column; the mobile phase used was 0.05 M aqueous ammonium acetate, acetonitrile and tetrahydrofuran (87:11:2, v/v) adjusted to pH 5.5 with acetic acid. The flow rate was 1 mL min(-1) and cefotaxime was quantified at 254 nm, with a sensitivity range of 0.005 AUFS. The validated method was specific, linear (R (2) a parts per thousand yen 0.999), precise and accurate in a concentration range of 0.2-50.0 mu g mL(-1). The method was rapid, selective and suitable for evaluation of cefotaxime in pH-sensitive Eudragit nanoparticles.

Language: English

Type (Professor's evaluation): Scientific