Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Rodrigues, F (Author) Other; Pereira, C (Author) Other; Pimentel, FB (Author) Other; Alves, RC (Author) Other; Ferreira, M (Author) Other; Sarmento, B (Author) Other; Helena Amaral, MH (Author) FFUP; Beatriz B P P Oliveira (Author) FFUP

Journal

Title: Industrial Crops and Products

Vol. 63

Pages: 167-174

ISSN: 0926-6690

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 31 Citations

Scientific classification

FOS: Agrarian Sciences > Agricultural biotechnology

Other information

Authenticus ID: P-00A-4SB

DOI: 10.1016/j.indcrop.2014.10.014

Abstract (EN): Recent changes in regulatory requirements and social views on animal testing have incremented the development of reliable alternative tests for predicting skin and ocular irritation potential of products based on new raw materials. In this regard, botanical ingredients used in cosmetic products are among those materials, and should be carefully reviewed concerning the potential presence of irritant constituents. In particular, cosmetic products used on the face, in vicinity of the eyes or that may come in contact with mucous membranes, should avoid botanical ingredients that contain, or are suspected to contain, such ingredients. In this study, we aimed to evaluate the effect of a new cosmetic ingredient, namely, coffee silverskin (CS), with an in vitro skin and ocular irritation assay using reconstructed human epidermis, EpiSkin (TM), and human corneal epithelial model, SkinEthics (TM) HCE, and an in vivo assay. Three different extracts of CS were evaluated. The histology of the models after extracts applications was analysed. The in vitro results demonstrated that extracts were not classified as irritant and the histological analyses proved that extracts did not affect both models structure. The content of caffeine, 5-hydroxymethyl furfural and chlorogenic acid was quantified after the epidermal assay. The in vivo test carried out with the most promising extract (hydroalcoholic) showed that, with respect to irritant effects, these extracts can be regarded as safe for topical application.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8