Alginate/Chitosan nanoparticles are effective for oral insulin delivery

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Sarmento, B (Author) Other; Ribeiro, A (Author) Other; Veiga, F (Author) Other; Sampaio, P (Author) Other; Neufeld, R (Author) Other; Ferreira, D (Author) FFUP

Journal

Title: Pharmaceutical Research

Vol. 24

Pages: 2198-2206

ISSN: 0724-8741

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 414 Citations

Scopus - 471 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-65A

DOI: 10.1007/s11095-007-9367-4

Abstract (EN): Purpose. To evaluate the pharmacological activity of insulin-loaded alginate/chitosan nanoparticles following oral dosage in diabetic rats. Methods. Nanoparticles were prepared by ionotropic pre-gelation of an alginate core followed by chitosan polyelectrolyte complexation. In vivo activity was evaluated by measuring the decrease in blood glucose concentrations in streptozotocin induced, diabetic rats after oral administration and flourescein (FITC)-labelled insulin tracked by confocal microscopy. Results. Nanoparticles were negatively charged and had a mean size of 750 nm, suitable for uptake within the gastrointestinal tract due to their nanosize range and mucoadhesive properties. The insulin association efficiency was over 70% and insulin was released in a pH-dependent manner under simulated gastrointestinal conditions. Orally delivered nanoparticles lowered basal serum glucose levels by more than 40% with 50 and 100 IU/kg doses sustaining hypoglycemia for over 18 h. Pharmacological availability was 6.8 and 3.4% for the 50 and 100 IU/kg doses respectively, a significant increase over 1.6%, determined for oral insulin alone in solution and over other related studies at the same dose levels. Confocal microscopic examinations of FITC-labelled insulin nanoparticles showed clear adhesion to rat intestinal epithelium, and internalization of insulin within the intestinal mucosa. Conclusion. The results indicate that the encapsulation of insulin into mucoadhesive nanoparticles was a key factor in the improvement of its oral absorption and oral bioactivity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9