Improving Oral Absorption of Samon Calcitonin by Trimyristin Lipid Nanoparticles

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Martins, S (Author) Other; Silva, AC (Author) Other; Ferreira, DC (Author) FFUP; Souto, EB (Author) Other

Journal

Title: Journal of Biomedical Nanotechnology

Vol. 5

Pages: 76-83

ISSN: 1550-7033

Publisher: American Scientific Publishers

Indexing

ISI Web of Knowledge - 47 Citations

Scopus - 53 Citations

Scientific classification

FOS: Engineering and technology > Nano-technology

Other information

Authenticus ID: P-003-NGP

DOI: 10.1166/jbn.2009.443

Abstract (EN): Solid lipid nanoparticles (SLN) composed of trimyristin (solid lipid) and poloxamer 407 (surfactant) were prepared by a w/o/w emulsion technique for the incorporation of Salmon calcitonin, and further explored as protein carriers for oral delivery. Trimyristin SLN showed a mean size diameter of 200 nm with an association efficiency for calcitonin of approx. 86%. The morphology of SLN was investigated by cryo-SEM and by AFM, revealing spheroid shape SLN with a smooth surface. The in vitro release of calcitonin occurred for a period of 8 h, under both gastric and intestinal simulated pH conditions, predicting suitable properties for oral administration. The pharmacological activity of the protein was evaluated following oral dosage of calcitonin-loaded SLN in rats. SLN lowered the basal blood calcium levels by up to 20% with 500 IU/kg dose sustaining hypocalcaemia over 8 h. The results indicate that incorporation of Salmon calcitonin into trimyristin SLN is a key factor for the improvement of the efficiency of such carriers for oral delivery of proteins.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8