Flavonoids and phenolic acids of sage: Influence of some agricultural factors

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Areias, F (Author) Other; Valentao, P (Author) Other; Andrade, PB (Author) FFUP; Ferreres, F (Author) Other; Seabra, RM (Author) Other

Journal

Title: Journal of Agricultural and Food Chemistry

Vol. 48

Pages: 6081-6084

ISSN: 0021-8561

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 59 Citations

Scopus - 68 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-000-YA4

DOI: 10.1021/jf000440+

Abstract (EN): A reversed-phase high-performance liquid chromatography/diode-array detector procedure is proposed for the determination of six phenolic compounds (caffeic acid, luteolin 7-O-glucoside, rosmarinic acid, apigenin, hispidulin, and cirsimaritin) in sage. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS2 (5-mum particle size, 25.0 x 0.46 cm) column. Of the several extractive solvents assayed only ethyl ether, ethyl acetate, and acetone were able to extract all the compounds mentioned. Best resolution was obtained using a gradient of water/phosphoric acid (999:1) and acetonitrile. Ten samples cultivated in two experimental fields (1997-1999) were analyzed and the individual compounds quantified. Four commercially available samples were also analyzed and the results are discussed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4