Vortex-assisted liquid-liquid micro-extraction and high-performance liquid chromatography for a higher sensitivity methyl methacrylate determination in biological matrices

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Tiago F A Sousa (Author) Other; Marta C Aniceto (Author) Other; Amorim, CG (Author) FFUP; Mariana Souto Lopes (Author) Other; Daniel Perez Mongiovi (Author) Other; Maria C B S M Montenegro (Author) FFUP; Alberto N Araujo (Author) FFUP

Journal

Title: Biomedical Chromatography

Vol. 28

Pages: 680-685

ISSN: 0269-3879

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 6 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-009-B0Z

DOI: 10.1002/bmc.3089

Abstract (EN): A vortex-assisted liquid-liquid micro-extraction coupled with high-performance liquid chromatography, with UV-vis, is proposed to pre-concentrate methyl methacrylate and to improve separation in biological matrices. The use of 1-octanol as extracting phase, its volume, the need for a dispersant agent, the agitation conditions and the cooling time before phase separation were evaluated. In optimum conditions, enrichment factors of 20 (+/- 0.5) and enrichment recovery of 99% were obtained. The straightforward association of this extraction process with the HPLC method, previously regulated by the International Organization for Standardization, afforded a detection limit of 122 ng/mL and a quantification limit of 370 ng/mL. The within-batch precision, relative standard deviation, was 3% for a sample with 1.49 mu g/mL and 4% for a sample with 13.4 mu g/mL. The results showed a between batch-precision of 21% for experiments performed on five different days, for a sample with a concentration of 1.10 mu g/mL in methyl methacrylate. Copyright (c) 2013 John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6