Screening of Antioxidant Phenolic Compounds Produced by In Vitro Shoots of Brassica oleracea L. var. costata DC

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Federico Ferreres (Author) Other; Carla Sousa (Author) Other; David M Pereira (Author) FFUP; Patricia Valentao (Author) FFUP; Marcos Taveira (Author) Other; Anabela Martins (Author) Other; Jose A Pereira (Author) Other; Rosa M Seabra (Author) Other; Paula B Andrade (Author) FFUP

Journal

Title: Combinatorial Chemistry & High Throughput Screening

Vol. 12

ISSN: 1386-2073

Publisher: Bentham Science

Indexing

ISI Web of Knowledge - 9 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-003-MPN

Abstract (EN): The phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC were screened by HPLC-DAD-MS/MS. Thirty seven compounds were characterized, which included chlorogenic acids, flavonoids (the majority of them were hydroxycinnamic acid esters of kaempferol and quercetin glycosides) and hydroxycinnamic acyl glycosides (with predominance of synapoyl gentiobiosides). The antioxidant capacity of the shoots was assessed against DPPH radical and two reactive oxygen species (superoxide radical and hypochlorous acid). A strong concentration-dependent antioxidative capacity was verified in the DPPH and superoxide radicals assays, but a reduced effect was noticed against hypochlorous acid. The results obtained indicate that the in vitro production of B. oleracea var. costata shoots can become important in the obtention of a noticeable dietary source of compounds with health protective potential.

Language: English

Type (Professor's evaluation): Scientific