Anti-Inflammatory Effect of Unsaturated Fatty Acids and Ergosta-7,22-dien-3-ol from Marthasterias glacialis: Prevention of CHOP-Mediated ER-Stress and NF-kappa B Activation

• Type: Article in International Scientific Journal
• Date: 2014

Authors

David M Pereira (Author) FFUP; Georgina Correia da Silva (Author) FFUP; Patricia Valenta (Author) FFUP; Natercia Teixeira (Author) FFUP; Paula B Andrade (Author) FFUP

Journal

Title: PLoS ONE

Vol. 9 No. 2

Final page: e88341

ISSN: 1932-6203

Publisher: Public Library of Science

Indexing

ISI Web of Knowledge - 30 Citations

Scopus - 43 Citations

Other information

Authenticus ID: P-009-710

DOI: 10.1371/journal.pone.0088341

Abstract (EN): There has been increasing awareness to the potential interest of drug discovery from marine natural products to treat several pathological conditions, including inflammation. In this work we describe the anti-inflammatory activity of several compounds present in the echinoderm Marthasterias glacialis (spiny sea-star), using the inflammatory model RAW 264.7 cells challenged with LPS. Lipidomic profiling of the organism revealed two major classes of compounds: fatty acids and sterols. Among these, the predominant compounds cis 11-eicosenoic and cis 11,14 eicosadienoic acids and the unsaturated sterol ergosta-7,22-dien-3-ol were evaluated. The mechanism of action of the compounds was distinct as they modulated different levels of the inflammation pathway. Classical inflammatory markers, such as COX-2, iNOS, IL-6 and NF-kappa B, were evaluated. We also studied the contribution of the CHOP pathway-mediated ER-stress to the inflammatory process. Overall, the sterol ergosta-7,22-dien-3-ol was the most active compound, however maximum activity was obtained when all compounds were tested in combination, thus suggesting a potentially synergistic activity of both classes of metabolites. This work establishes the echinoderm M. glacialis as an interesting source of anti-inflammatory molecules.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9