Immunoreactive pattern of Staphylococcus epidermidis biofilm against human whole saliva

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Virgínia Carvalhais (Author) Other; Francisco Amado (Author) Other; Frederico Cerveira (Author) Other; Rita Ferreira (Author) Other; Manuel Vilanova (Author) ICBAS; Nuno Cerca (Author) Other; Rui Vitorino (Author) Other

Journal

Title: Electrophoresis

Vol. 36 No. 9-10

Pages: 1228-1233

ISSN: 0173-0835

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 3 Citations

ISI Web of Science

Scopus - 3 Citations

Pubmed / Medline

Scientific classification

FOS: Natural sciences > Biological sciences

CORDIS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-00G-4VP

DOI: 10.1002/elps.201500043

Abstract (EN): Saliva is essential to interact with microorganisms in the oral cavity. Therefore, the interest in saliva antimicrobial properties is on the rise. Here, we used an immunoproteomic approach, based on protein separation of Staphylococcus epidermidis biofilms by 2DE, followed by Western-blotting, to compare human serum and saliva reactivity profile. A total of 17 proteins were identified by MALDI-TOF/TOF. Serum and saliva presented a distinct pattern of immunoreactive proteins. Our results suggest that saliva seems to have higher propensity to react against S. epidermidis proteins with oxidoreductase activity and proteins involved with L-serine metabolic processes. We show that saliva was a powerful tool for the identification of potential S. epidermidis biofilms proteins.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6