Detection of prognostic significant translocations in childhood acute lymphoblastic leukaemia by one-step multiplex reverse transcription polymerase chain reaction

• Type: Article in International Scientific Journal
• Date: 2000

Authors

cerveira, n (Author) Other; ferreira, s (Author) Other; doria, s (Author) FMUP; veiga, i (Author) Other; ferreira, f (Author) Other; martz, jm (Author) Other; marques, m (Author) Other; castedo, s (Author) FMUP

Journal

Title: British Journal of Haematology

Vol. 109

Pages: 638-640

ISSN: 0007-1048

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 11 Citations

Scientific classification

FOS: Medical and Health sciences > Clinical medicine

Other information

Authenticus ID: P-000-ZSH

DOI: 10.1046/j.1365-2141.2000.02051.x

Abstract (EN): The search for chromosomal translocations in df novo cases of childhood acute lymphoblastic leukaemia (ALL) is crucial for the selection of the appropriate therapeutic protocol. In this work, we describe a new method - one-step multiplex reverse transcription polymerase chain reaction (RT-PCR) - to screen for prognostic significant translocations in childhood ALL. Our approach involves a single PCR reaction for the simultaneous detection of the molecular rearrangements resulting from the t(9:22), t(12;21), t(4;11) and t(1;19), with a turnaround time of less than 24 h. This assay proved to be highly sensitive, specific, reproducible and easy to implement in a routine genetics laboratory.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3