Growth, immune responses and intestinal morphology of rainbow trout (Oncorhynchus mykiss) supplemented with commercial probiotics

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Ramos, MA (Author) Other; goncalves, j (Author) ICBAS; Batista, S (Author) Other; Costas, B (Author) Other; Pires, MA (Author) Other; Rema, P (Author) Other; Ozorio, ROA (Author) Other

Journal

Title: Fish and Shellfish Immunology

Vol. 45

Pages: 19-26

ISSN: 1050-4648

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 18 Citations

ISI Web of Science

Scopus - 32 Citations

Scientific classification

FOS: Agrarian Sciences > Agriculture, Forestry, and Fisheries

Other information

Authenticus ID: P-00G-BQ9

DOI: 10.1016/j.fsi.2015.04.001

Abstract (EN): The influence of two commercial probiotics on the growth, innate immune parameters and intestinal morphology of rainbow trout (Oncorhynchus mykiss) juveniles (initial weight: 16.4 +/- 0.4 g) was evaluated. Two probiotic types: A, multi-species (Bacillus sp., Pedicoccus sp., Enterococcus sp., Lactobacillus sp.) and B, mono-species (Pediococcus acidilactici) were tested at two levels each (A(1): 1.5 g kg(-1), 8.6 x 10(5) CFU g(-1); A(2): 3 g kg(-1), 1.6 x 10(6) CFU g(-1); B-1: 0.1 g kg(-1), 2.6 x 10(4) CFU g(-1); B-2: 0.2 g kg(-1), 7.2 x 10(4) CFU g(-1)) versus an unsupplemented diet (C). Diets were distributed to sextuplicate tanks, three times a day to visual satiation for 8 weeks. Growth performance and immune responses (plasma lysozyme, ACH50, peroxidase and head kidney respiratory burst) were determined at 4 and 8 weeks of feeding. Body composition and intestine morphology were determined at the end of the feeding trial. At 8 weeks, the lower dose of multi-species probiotic (A(1)) improved growth rate, while both probiotic types improved feed conversion rate compared to the control animals, at the lower dose of multi-species (A(1)) and at the higher dose of mono-species (B-2) probiotics. Body composition did not vary between treatments. At 4 weeks, ACH50 activity was significantly higher in fish fed higher dose of B probiotic (B-2, 123.7 +/- 50.6 vs 44.1 +/- 7.7 U.ml(-1) in control). At 8 weeks, lysozyxne activity was higher in fish fed A(1) (13.1 +/- 5.2 mu g ml(-1)) diet compared to fish fed control diet (7.8 +/- 1 mu g ml(-1)). Plasma peroxidase and head-kidney respiratory burst did not differ among the dietary treatments. Villi length and integrity and goblet cell counting of a cross section of the anterior intestine were not significantly different between groups. Results suggest benefits in zootechnical performance and immune humoral responses using both probiotic types, in a dose dependent manner, without apparent alterations in intestinal morphology.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8