Anti-Candida Activity of a Chitosan Hydrogel: Mechanism of Action and Cytotoxicity Profile

• Type: Article in International Scientific Journal
• Date: 2010

Authors

palmeira-de-oliveira, a (Author) Other; ribeiro, mp (Author) Other; palmeira-de-oliveira, r (Author) Other; gaspar, c (Author) Other; costa-de-oliveira, s (Author) FMUP; correia, ij (Author) Other; vaz, cp (Author) FMUP; martinez-de-oliveira, j (Author) Other; queiroz, ja (Author) Other; rodrigues, ag (Author) FMUP

Journal

Title: Gynecologic and Obstetric Investigation

Vol. 70

Pages: 322-327

ISSN: 0378-7346

Publisher: Karger

Indexing

ISI Web of Knowledge - 41 Citations

Scopus - 42 Citations

Scientific classification

FOS: Medical and Health sciences > Clinical medicine

Other information

Authenticus ID: P-00H-ZRD

DOI: 10.1159/000314023

Abstract (EN): Candida spp are common causative agents of mucocutaneous infections New therapeutic antifungal drugs are needed to treat chronic disease as these are frequently clinically resistant to azols Chitosan, among other possible vehicles for active compounds, shows an added value as it appears to have intrinsic antimicrobial properties The aim of the present study was to evaluate the anti-Candida activity of a medium-molecular-weight chitosan hydrogel (CH), to clarify its possible mechanism of action and to evaluate its cytotoxicity on human fibroblasts CH antifungal activity was assessed according to CLSI reference M27-A3 protocol, its mechanism of action was investigated by flow cytometry, and its cytotoxicity was studied by MTT assay CH demonstrated a full inhibition of C tropical's, C kruser, C guilliermondir and C parapsilosis growth while impairing C albicans and C glabrata viability Flow cytometry tests showed that CH acts by inducing primary lesion of the cytoplasmic membrane However, CH showed no cytotoxic effect upon human fibroblasts cells Resistant strains will require new therapeutic approaches Chitosan being a good carrier and having itself anti-Candida activity seems to be a promising vehicle to be used for the treatment of mucocutaneous candidosis Copyright (c) 2010 S Karger AG Basel

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6