Assessment of cardiovascular disease risk using immunosensors for determination of C-reactive protein levels in serum and saliva: a pilot study

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Celine I L Justino (Author) Other; Katia Duarte (Author) Other; Susana Lucas (Author) Other; Paulo Chaves (Author) Other; Paulo Bettencourt (Author) Other; Ana Cristina Freitas (Author) Other; Ruth Pereira (Author) FCUP; Susana Cardoso (Author) Other; Armando C Duarte (Author) Other; Teresa A P Rocha Santos (Author) Other

Journal

Title: Bioanalysis

Vol. 6

Pages: 1459-1470

ISSN: 1757-6180

Publisher: Future Medicine Ltd.

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 15 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-009-Q8F

DOI: 10.4155/bio.14.12

Abstract (EN): Background: Disposable immunosensors based on field effect transistors with single-walled carbon nanotubes (NTFET) were applied for the first time to clinical samples of undiluted blood serum and saliva for the determination of C-reactive protein (CRP), and validated by comparison with ELISA. Results: The NTFET showed comparable analytical performance with the ELISA when applied to clinical samples, which means that NTFET can be used as an alternative to ELISA. Also, a high correlation between the serum and salivary CRP levels was found with the NTFET, which means that saliva could be used based on a noninvasive sampling as an alternative fluid to blood serum. The establishment of a new range of CRP levels based on saliva was also found. Conclusion: The monitoring of CRP in saliva samples by disposable immunosensors could be a valuable approach for the improvement of healthcare services, considering the worldwide increased incidence of cardiovascular diseases.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12