An immunoproteomic approach for characterization of dormancy within Staphylococcus epidermidis biofilms

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Virgínia Carvalhais (Author) Other; Frederico Cerveira (Author) Other; Manuel Vilanova (Author) Other; Nuno Cerca (Author) Other; Rui Vitorino (Author) Other

Journal

Title: Molecular Immunology

Vol. 65 No. 2

Pages: 429-435

ISSN: 0161-5890

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

ISI Web of Science

Scopus - 11 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Basic medicine

CORDIS: Health sciences > Medical sciences > Medicine > Immunology

Other information

Authenticus ID: P-00A-994

DOI: 10.1016/j.molimm.2015.02.024

Abstract (EN): Virulence of Staphylococcus epidertnidis is mainly attributed to surface colonization and biofilm formation in indwelling medical devices. Physiological heterogeneity of biofilms may influence host immune response and sensitivity to antibiotics. Dormant cells, among others, contribute to biofilm heterogeneity. The aim of this study was to identify immunogenic proteins of S. epidermidis biofilms associated with dormancy mechanism, by using two-dimensional electrophoresis (2-DE) immunoblotting and mass spectrometry (MS). A total of 19 bacterial proteins, recognized by human serum samples, were identified. These proteins were mainly involved in small molecule metabolic biological processes. Catalytic activity and ion binding were the most representative molecular functions. CodY and GpmA proteins were more reactive to sera when biofilm dormancy was induced, while FtnA and ClpP were more reactive when dormancy was prevented. This is the first work that identifies differences in immunoreactive proteins within bacterial biofilms with induced or prevented dormancy. Considering the importance of dormancy within biofilms, further evaluation of these proteins can provide insights into the mechanisms related to dormancy and help to improve current understanding on how dormancy affects the host immune response.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7