Recovery rate, morphological canine cumulus-oocyte quality and nuclear maturity of complexes collected from anestrous or diestrous bitches of different ages

• Type: Article in International Scientific Journal
• Date: 2007

Authors

lopes, g (Author) ICBAS; sousa, m (Author) ICBAS; luvoni, gc (Author) Other; rocha, a (Author) ICBAS

Journal

Title: Theriogenology

Vol. 68

Pages: 821-825

ISSN: 0093-691X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 8 Citations

Scientific classification

FOS: Agrarian Sciences > Veterinary science

Other information

Authenticus ID: P-004-7CH

DOI: 10.1016/j.theriogenology.2007.06.011

Abstract (EN): Canine cumulus-oocyte complexes (COC) were recovered from ovaries of post-pubertal animals (1-3, 4-6 and 7-10 years old) at different ovarian estrous phases (anestrus and diestrus). The number of COCs, and the number and nuclear maturity of high-quality (grade- 1) oocytes were assessed. For all animals, no significant differences were found between the two reproductive phases relatively to the total number of COCs and grade-1 oocytes recovered. However, significant higher numbers of COCs were recovered from young than from elderly animals, and the proportion of grade- I oocytes was also significantly higher in the younger group than in the other two age-groups. Of 226 grade-1 oocytes, 73% were at the germinal vesicle stage (GV), 10% had resumed meiosis (9% at germinal vesicle breakdown; 1% at metaphase-I) and 17% were degenerated. A significant effect of the reproductive phase on oocyte nuclear maturity was found only for adult animals, with a higher number of GV oocytes being found at anestrous (79%) due to higher rates of meiosis resumption (34%) at diestrous. The high number of grade- I oocytes with meiosis resumption and fragmented or unidentified nuclear contents, indicates that current criteria for the selection of viable canine COCs are not optimized and need a new definition.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5