Renalase regulates peripheral and central dopaminergic activities

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Quelhas Santos, J (Author) FMUP; Serrao, MP (Author) Other; Soares Silva, I (Author) Other; Fernandes Cerqueira, C (Author) Other; Simoes Silva, L (Author) Other; Pinho, MJ (Author) Other; Remiao, F (Author) FFUP; Sampaio Maia, B (Author) FMUP; Desir, GV (Author) Other; Pestana, M (Author) Other

Journal

Title: American Journal of Physiology - Renal Physiology

Vol. 308

Pages: F84-F91

ISSN: 1931-857X

Publisher: American Physiological Society

Indexing

ISI Web of Knowledge - 15 Citations

ISI Web of Science

Scopus - 18 Citations

Pubmed / Medline

Scientific classification

CORDIS: Health sciences

Other information

Authenticus ID: P-00A-45Z

DOI: 10.1152/ajprenal.00274.2014

Abstract (EN): Renalase is a recently identified FAD/NADH-dependent amine oxidase mainly expressed in kidney that is secreted into blood and urine where it was suggested to metabolize catecholamines. The present study evaluated central and peripheral dopaminergic activities in the renalase knockout (KO) mouse model and examined the changes induced by recombinant renalase (RR) administration on plasma and urine catecholamine levels. Compared with wild-type (WT) mice, KO mice presented increased plasma levels of epinephrine (Epi), norepinephrine (NE), and dopamine (DA) that were accompanied by increases in the urinary excretion of Epi, NE, DA. In addition, the KO mice presented an increase in urinary DA-to-L-3,4-dihydroxyphenylalanine (L-DOPA) ratios without changes in renal tubular aromatic-L-amino acid decarboxylase (AADC) activity. By contrast, the in vivo administration of RR (1.5 mg/kg sc) to KO mice was accompanied by significant decreases in plasma levels of Epi, DA, and L-DOPA as well as in urinary excretion of Epi, DA, and DA-to-L-DOPA ratios notwithstanding the accompanied increase in renal AADC activity. In addition, the increase in renal DA output observed in renalase KO mice was accompanied by an increase in the expression of the L-type amino acid transporter like (LAT) 1 that is reversed by the administration of RR in these animals. These results suggest that the overexpression of LAT1 in the renal cortex of the renalase KO mice might contribute to the enhanced L-DOPA availability/uptake and consequently to the activation of the renal dopaminergic system in the presence of renalase deficiency.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8