Ultrastructure and germination of Vitis vinifera cv. Loureiro pollen

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Abreu, I (Author) FCUP; Costa, I (Author) Other; Oliveira, M (Author) Other; Cunha, M (Author) FCUP; de Castro, R (Author) Other

Journal

Title: Protoplasma

Vol. 228

Pages: 131-135

ISSN: 0033-183X

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 25 Citations

Scopus - 25 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-004-JD5

DOI: 10.1007/s00709-006-0167-1

Abstract (EN): The cultivar Loureiro of Vitis vinifera is one of the most economically important, recommended in almost the totality of the Regiao Demarcada dos Vinhos Verdes. In vineyards, the grape productivity of this cultivar is normal while in others it is extremely low. The aim of this work was to study the morphology and germination of Vitis vinifera cv. Loureiro pollen with high and low productivity. The pollen grain was examined under light, transmission and scanning electron microscopy. Typically V. vinifera pollen present three furrows but in the cultivar Loureiro we found tricolporated and acolporated (without furrows or pores) pollen grains. Both pollen types present generative and vegetative cells with the usual aspect and a dense cytoplasm rich in organelles. In the acolporated pollen a continuous exine layer and an irregular intine layer were observed. Differences were found in the starch accumulation, since only in tricolporated pollen abundant plastids filled with numerous starch granules were observed. To determine the causes of the low productivity of this cultivar we tested pollen viability by the fluorochromatic reaction and pollen germinability by in vitro assays. We observed that the acolporated pollen grain is viable, but no germination was recorded.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5