Go to:
Logótipo
Comuta visibilidade da coluna esquerda
Você está em: Start > Project/Service Agreement:POCI-01-0145-FEDER-030431

Project/Service Agreement:POCI-01-0145-FEDER-030431

Start Approved In Progress Completed Closed

Status
Projeto EncerradoClosed
Publication
PublicadoPublished
General Data
Code: 72994
 
Reference: POCI-01-0145-FEDER-030431
Short name: CLASInVivo
Title: Development of NAM-CLASI-FIVH method: a new in vitro tool for an accurate analysis of diversity and spatial organization of gastric microbiota
Competitive Funding: Yes
Does it involve businesses?: No
No. of Participating Institutions: 2
Scope
Type: Funded Project
 
Geographical Scope: National
 
Type of Action: R&TD
Funding
Programme: P2020|COMPETE - Projetos em Todos os Domínios Científicos
Funding Institution: FCT - Fundação para a Ciência e a Tecnologia
Financial Geographical Scope: National
Date of the Funding Agreement: 2018-05-30
Paying Entity: Fundação para a Ciência e a Tecnologia
Scheduling
Effective Start Date: 2018-07-26
Expected Completion Date: 2022-07-25
Effective Completion Date: 2022-07-25
Budget
Currency: EUR
 
Total Approved Budget: 186.303,47 EUR
Details
Summary: Helicobacter pylori is the main cause of several gastroduodenal diseases, but recent data showed the presence of
non-H. pylori bacteria in the gastric mucosa and gastric juice, which means that interactions between non-H. pylori
population and H. pylori are possible, if not likely. Hence, the possible impact of the microbiota in gastric diseases
needs to be defined.
To clarify this paradigm, the Fluorescence In Vivo Hybridization (FIVH), was recently applied directly in the stomach,
but this detection was limited to a single bacterium (H. pylori). In addition, as the number of microorganisms that
can be discriminated by conventional FISH technique is usually limited to 3 targets, another new technology, called
Combinatorial Labeling and Spectral Imaging-FISH (CLASI-FISH), is emerging. However, these FISH techniques
suffer from limitations associated to the use of DNA probes, which might be overcome taking advantage of the
enhanced hybridization properties of nucleic acid mimics (NAMs). Taking into account this, the main goal of this
proposal is to combine the FIVH and CLASI-FISH using NAMs probes (NAM-CLASI-FIVH) in order to create a more
straightforward procedure to study the composition and spatial organization of the microbiota directly in the stomach.
In future, the application of the method together with sotisfied equipments (e.g. confocal laser endomicroscopy) will
then be useful to track over time the gastric microbiota behavior directly in the stomach of animal models without
sacrificed them.
First, to accomplish our goal, the NAMs probes used in NAM-CLASI-FIVH technique will be developed and optimized
in conditions similar those found in stomach (e.g. low pH, 37°C); then, the technique will be validated in gastric biopsies
samples. Once this has been achieved, the developed protocol will be tested and adpated in a mouse model. Then,
as a case study, we will analyse the microbial changes over time in a mouse model in terms of composition Ver mais. Adequado para parcelas de texto incompletas e que, através deste ícone, permite-se que o utilizador leia o texto todo.
Scientific Context
Scientific Domain (FOS - Level 2): Engineering and technology

Academic fields (CORDIS - Level 5)

Mais informações There are no Academic Fields associated with the Project.

Keywords

Mais informações There are no Keywords associated with the Project.
Documents
Mais informações There are no Documents associated with the Project.

Publications associated with the Project

Article in International Scientific Journal
Andreia S. Azevedo (Author) (FEUP); Inês M. Sousa (Author) (Other); Ricardo M. Fernandes (Author) (Other); Nuno F. Azevedo (Author) (FEUP); Carina Almeida (Author) (FEUP)
2019
Institutions Participating in the Project
Institution Contact Create Tab?
Name Short name Country Type Participation Name Telephone Email
Faculdade de Engenharia da Universidade do Porto FEUP Portugal University Proponent
Instituto de Patologia e Imunologia Molecular da Universidade do Porto IPATIMUP Portugal RD Institute Partner
 
Budgets and Teams
Approved Budget: 159.245,97 EUR
Approved Funded Amount: 159.245,97 EUR
Approved co-funded Amount: 0,00 EUR
Funding Rate: 100 %
Confidential Budget:

People in the Project

Institution Name Short name Role Dedication (%) Contribution (%) Allocation
Start date End date
FEUP Andreia Sofia Mateus Azevedo ASMA Official Researcher at the OU
FEUP Andreia Sofia Mateus Azevedo ASMA Researcher 100 2019-01-28 2021-12-31
FEUP Nuno Filipe Ribeiro Pinto de Oliveira Azevedo NFA Researcher
FEUP Raquel Claro Silva Carvalho Sousa RCSCS Grant recipient 100 2019-11-18 2020-12-17
FEUP Rita Sobral Fernandes Machado dos Santos RSFMS Researcher

Technicians in the Project

Technician Contact
FEUP 423364 Maria Isabel Esteves Peres 220413579
Laboratories
Mais informações There are no Laboratories associated with the Project.
Recommend this page Top
Copyright 1996-2025 © Faculdade de Direito da Universidade do Porto  I Terms and Conditions  I Acessibility  I Index A-Z
Page created on: 2025-07-09 at 22:59:12 | Privacy Policy | Personal Data Protection Policy | Whistleblowing