Coaggregation dynamics in drinking water biofilms and implications for chlorine disinfection

• Type: Article in International Scientific Journal
• Date: 2024-09

Authors

Inês Gomes (Author) FEUP; Ana C. Afonso (Author) Other; Francisca Massano (Author) Other; Maria J. Saavedra (Author) Other; Lúcia C. Simões (Author) Other; Simões M (Author) FEUP

Journal

Title: Journal of Hazardous Materials

Vol. 480

Pages: 135948-135948

ISSN: 0304-3894

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

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Other information

Authenticus ID: P-017-478

DOI: 10.1016/j.jhazmat.2024.135948

Abstract (EN): Biofilms in drinking water (DW) systems persistently challenge traditional disinfection methods due to intricate microbial interactions, with coaggregation playing a crucial role in forming multispecies biofilms. This study examined the implications of coaggregation on tolerance towards sodium hypochlorite (NaOCl) disinfection. Dual-species biofilms were formed for seven days on polyvinyl chloride coupons, comprising a strain of the emerging pathogen Stenotrophomonas maltophilia and the coaggregating strain Delftia acidovorans 005 P. For comparison, dual-species biofilms were also formed with a non-coaggregation strain (D. acidovorans 009 P). The minimum bactericidal concentration (MBC) for each planktonic strain varied (D. acidovorans: 1 mg/L, S. maltophilia: 1.5 mg/L) below the safe DW treatment limits. However, high NaOCl doses (10 xMBC and 100 xMBC,) showed low efficacy against dual-species biofilms, indicating significant biofilm tolerance to disinfection. Membrane damage occurred at sub-MBC without culturability loss, underscoring biofilm resilience. The biofilm analysis revealed a complex interplay between the composition of extracellular polymeric substances and the architecture, which was influenced by the presence of the coaggregating strain. Overall, coaggregation significantly influenced biofilm formation and resilience, impacting NaOCl disinfection. These findings underscore the challenges of microbial interactions in biofilms, emphasizing the need for improved disinfection strategies to control biofilms in drinking water systems.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9