Direct and indirect effects of P(2)O(5) glass reinforced-hydroxyapatite composites on the growth and function of osteoblast-like cells

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Maria Ascensão Lopes (Author) FEUP; J. C. Knowles (Author) Other; José Domingos Santos (Author) FEUP; Fernando Jorge Monteiro (Author) FEUP; I. Olsen (Author) Other

Journal

Title: Biomaterials

Vol. 21 No. 11

Pages: 1165-1172

ISSN: 0142-9612

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 31 Citations

ISI Web of Science

Scopus - 34 Citations

COMPENDEX

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

CORDIS: Technological sciences > Engineering > Biomaterial engineering ; Technological sciences > Engineering > Biomedical enginnering

Other information

Authenticus ID: P-000-ZSA

DOI: 10.1016/s0142-9612(00)00007-7

Abstract (EN): Human osteoblast-like cells were plated on hydroxyapatite and P(2)O(5)-glass reinforced hydroxyapatite composite discs. They were also cultured in the presence of media obtained by incubating the discs in the absence of cells. The effects of these direct and indirect interactions were examined by measuring cell proliferation and the expression of certain key extracellular matrix antigens. One composite was found to initially delay cell growth, while the extract of a different composite appeared to down-regulate DNA synthesis. Flow cytometry analysis showed that growth directly on the discs had little effect on collagen type I, but reduced fibronectin and osteocalcin levels. The extracts of the materials generally had less effect, although one extract obtained from the glass-reinforced hydroxyapatite significantly down-regulated fibronectin. These in vitro studies thus suggest that there were only few differences overall in the growth of the cells directly on the glass-reinforced compared with the hydroxyapatite discs and also only relatively small effects of the extracts on the cells. However, the flow cytometry results suggest that both the materials and the extracts may have a potentially important influence on connective tissue production, and that these effects are both material- and antigen-specific.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8