Oral bioavailability of insulin contained in polysaccharide nanoparticles

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Bruno Sarmento (Autor) Outra; Antonio Ribeiro (Autor) Outra; Francisco Veiga (Autor) Outra; Domingos Ferreira (Autor) FFUP; Ronald Neufeld (Autor) Outra

Revista

Título: Biomacromolecules

Vol. 8

Páginas: 3054-3060

ISSN: 1525-7797

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 163 Citações

Scopus - 212 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-004-6XG

DOI: 10.1021/bm0703923

Abstract (EN): The pharmacological activity of insulin-loaded dextran sulfate/chitosan nanoparticles was evaluated following oral dosage in diabetic rats. Nanoparticles were mucoadhesive and negatively charged with a mean size of 500 nm, suitable for uptake within the gastrointestinal tract. Insulin association efficiency was over 70% and was released in a pH-dependent manner under simulated gastrointestinal conditions. Orally delivered nanoparticles lowered basal serum glucose levels in diabetic rats around 35% with 50 and 100 IU/kg doses sustaining hypoglycemia over 24 h. Pharmacological availability was 5.6 and 3.4% for the 50 and 100 IU/kg doses, respectively, a significant increase over 1.6%, determined for oral insulin alone in solution. Confocal microscopic examinations of FITC-labeled insulin nanoparticles showed adhesion to rat intestinal epithelium, and internalization of insulin within the intestinal mucosa. Encapsulation of insulin into dextran sulfate/chitosan nanoparticles was a key factor in the improvement of the bioavailability of its oral delivery over insulin solution.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7